1H93
ACTIVE MUTANT (S215->C) OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM LEUCONOSTOC MESENTEROIDES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11-12 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.330, 44.280, 91.170 |
| Unit cell angles | 90.00, 105.39, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SUBUNIT 'A' OF 1DPG |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.460 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.081 * | 0.298 * |
| Total number of observations | 57502 * | |
| Number of reflections | 24710 | 2148 * |
| <I/σ(I)> | 12.6 | 2.9 |
| Completeness [%] | 96.4 | 85.2 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 * | HANGING DROP VAPOUR DIFFUSION, 2+2 MICROLITER DROPS. THE WELL BUFFER: 20% V/V PEG 400 IN 0.1M HEPES-NAOH, PH 7.5 WITH 0.2M CALCIUM CHLORIDE. THE PROTEIN AT 10MG/ML IN 100MM TRIS-HCL WITH 12.5MM NAD+. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 20 (%(v/v)) | |
| 2 | 1 | reservoir | HEPES-NaOH | 0.1 (M) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | Tris-HCl | 100 (mM) | |
| 6 | 1 | drop | NAD+ | 12.5 (mM) |
