1H8U
Crystal Structure of the Eosinophil Major Basic Protein at 1.8A: An Atypical Lectin with a Paradigm Shift in Specificity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.328, 57.487, 60.961 |
| Unit cell angles | 90.00, 113.20, 90.00 |
Refinement procedure
| Resolution | 36.180 - 1.800 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lit |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 * | 0.229 * |
| Total number of observations | 217030 * | |
| Number of reflections | 21127 | |
| <I/σ(I)> | 16.2 | 5.18 |
| Completeness [%] | 96.0 | 91.8 |
| Redundancy | 10.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 20 * | 100MM MALONIC ACID PH6, 140MM POTTASIUM DIHYDROGEN PHOSPHATE., pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | reservoir | malonic acid | 100 (mM) | |
| 3 | 1 | reservoir | potassium phosphate | 160 (mM) | |
| 4 | 1 | reservoir | L-DTT | 5 (mM) | |
| 5 | 1 | reservoir | sodium acetate | 160 (mM) |
