1H8U

Crystal Structure of the Eosinophil Major Basic Protein at 1.8A: An Atypical Lectin with a Paradigm Shift in Specificity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-15
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	74.328, 57.487, 60.961
Unit cell angles	90.00, 113.20, 90.00

Refinement procedure

Resolution	36.180 - 1.800
R-factor	0.234
Rwork	0.234
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lit
RMSD bond length	0.009
RMSD bond angle	24.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.070 *	0.229 *
Total number of observations	217030 *
Number of reflections	21127
<I/σ(I)>	16.2	5.18
Completeness [%]	96.0	91.8
Redundancy	10.27

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	20 *	100MM MALONIC ACID PH6, 140MM POTTASIUM DIHYDROGEN PHOSPHATE., pH 6.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	16 (mg/ml)
2	1	reservoir	malonic acid	100 (mM)
3	1	reservoir	potassium phosphate	160 (mM)
4	1	reservoir	L-DTT	5 (mM)
5	1	reservoir	sodium acetate	160 (mM)