1H8P
Bull seminal plasma PDC-109 fibronectin type II module
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.07466,1.07516,0.8856, 1.05271 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 90.881, 90.881, 52.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 1.800* |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.23000 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.920 |
| High resolution limit [Å] | 1.800 * | 1.820 |
| Rmerge | 0.032 * | 0.119 * |
| Total number of observations | 275885 * | |
| Number of reflections | 22445 * | |
| <I/σ(I)> | 11.7 | 5.3 |
| Completeness [%] | 99.0 | 96.3 |
| Redundancy | 5.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 * | 22 * | CRYSTALS WERE OBTAINED IN 30% ISOPROPANOL, 5% PEG 4000, 0.1 M HEPES, PH 7.2. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | o-phosphorylcholine | 50 (mg/ml) | |
| 3 | 1 | reservoir | isopropanol | 30 (%(v/v)) | |
| 4 | 1 | reservoir | PEG4000 | 8 (%(w/v)) | |
| 5 | 1 | reservoir | imidazole | 0.1 (M) | pH8.0 |
