1H8K
A-SPECTRIN SH3 DOMAIN A11V, V23L, M25V, V53I, V58L MUTANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE M18X |
Temperature [K] | 100 |
Collection date | 2000-11-15 |
Detector | SMALL MARRESEARCH IMAGING PLATE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.510, 33.236, 62.609 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.700 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1shg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.850 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 | 2.820 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.110 | |
Number of reflections | 2863 | |
Completeness [%] | 88.8 | 71.3 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | PROTEIN WAS CRYSTALLIZED FROM 1.1 M AMMONIUM SULPHATE, 0.1M ACETATE/AC. ACETIC, PH=4.5, 90 MM BIS-TRIS PROPANE, 0.9 MM SODIUM AZIDE, pH 4.50 |