1H89
CRYSTAL STRUCTURE OF TERNARY PROTEIN-DNA COMPLEX2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-17 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.684, 73.383, 161.699 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.450 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h88 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.120 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.072 * | |
| Total number of observations | 205777 * | |
| Number of reflections | 27451 | |
| <I/σ(I)> | 27.5043 | 2.162 |
| Completeness [%] | 99.0 | 95.5 |
| Redundancy | 7.496 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 * | 297 | Tahirov, T.H., (2001) Acta Crystallogr.,Sect.D, 57, 1655. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dithiothreitol | 10 (mM) | pH6.8 |
| 2 | 1 | drop | DNA | 10 (%) | excess |
| 3 | 1 | drop | protein | 6.5-7.5 (mg/ml) | |
| 4 | 1 | reservoir | 0.1 (M) | ||
| 5 | 1 | reservoir | 0.005 (M) | ||
| 6 | 1 | reservoir | sodium HEPES | 0.05 (M) | pH7.0 |
| 7 | 1 | reservoir | MPD | 4 (%(v/v)) | |
| 8 | 1 | reservoir | glycerol | 6 (%(v/v)) |
