1H88
CRYSTAL STRUCTURE OF TERNARY PROTEIN-DNA COMPLEX1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-25 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.735, 156.966, 55.868 |
| Unit cell angles | 90.00, 100.16, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.800 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.27700 |
| Structure solution method | MAD AND MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.140 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.9) |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.046 * | |
| Total number of observations | 56631 * | |
| Number of reflections | 18605 | |
| <I/σ(I)> | 25.2564 | 2.109 |
| Completeness [%] | 96.0 | 87.8 |
| Redundancy | 3.151 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 * | 297 | Tahirov, T.H., (2001) Acta Crystallogr.,Sect.D, 57, 1655. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dithiothreitol | 10 (mM) | pH6.8 |
| 2 | 1 | drop | DNA | 10 (%) | excess |
| 3 | 1 | drop | protein | 6.5-7.5 (mg/ml) | |
| 4 | 1 | reservoir | ammonium acetate | 0.05 (M) | |
| 5 | 1 | reservoir | 0.01 (M) | ||
| 6 | 1 | reservoir | Tris-HCl | 0.05 (M) | pH7.5 |
| 7 | 1 | reservoir | MPD | 10 (%(v/v)) | |
| 8 | 1 | reservoir | glycerol | 6 (%(v/v)) |
