1H85

FERREDOXIN:NADP+ REDUCTASE MUTANT WITH VAL 136 REPLACED BY LEU (V136L)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	2000-05-15
Detector	MARRESEARCH
Spacegroup name	P 65
Unit cell lengths	86.650, 86.650, 96.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.21
Rwork	0.210
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1que
RMSD bond length	0.011
RMSD bond angle	1.058
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.500	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.100	0.260
Number of reflections	18325
<I/σ(I)>	8	3.1
Completeness [%]	99.4	96.4
Redundancy	12.6	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		pH 5.00