1H80

1,3-ALPHA-1,4-BETA-D-GALACTOSE-4-SULFATE- 3,6-ANHYDRO-D-GALACTOSE-2-SULFATE 4 GALACTOHYDROLASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-07-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	55.870, 90.070, 124.120
Unit cell angles	90.00, 93.53, 90.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.207
Rwork	0.207
R-free	0.22300
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	26.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.123 *	0.203 *
Total number of observations	157467 *
Number of reflections	762046
Completeness [%]	97.7	96.1
Redundancy	4.80 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	288 *	Michel, G., (2000) Acta Crystallogr.,Sect.D, 56, 766. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)	pH7.2
3	1	drop		200 (mM)
4	1	drop	dithiothreitol	1 (mM)