1H7Z

Adenovirus Ad3 fibre head

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-08-15
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	96.580, 96.580, 154.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.960 - 1.600
R-factor	0.176 *
Rwork	0.176
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1knb
RMSD bond length	0.015
RMSD bond angle	0.035
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.389	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.045	0.331
Number of reflections	108500
<I/σ(I)>	9	1.9
Completeness [%]	98.6	97.9
Redundancy	3.1	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		RESERVOIR: 1.5 M LI2SO4, 0.1 M HEPES PH 7.0, 7.5 MG/ML PROTEIN IN 0.02 M TRIS-HCL PH 7.0 0.001 M EDTA, 0.02 M NACL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		1.25 (M)	or 1.5M
2	1	reservoir	HEPES	0.1 (M)
3	1	drop	protein	7.5 (mg/ml)