1H7B
Structural basis for allosteric substrate specificity regulation in class III ribonucleotide reductases, native NRDD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-12-01 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 98.019, 98.019, 242.421 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.450 |
R-factor | 0.229 * |
Rwork | 0.224 |
R-free | 0.26000 * |
Structure solution method | MIR |
RMSD bond length | 0.013 * |
RMSD bond angle | 23.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.460 |
High resolution limit [Å] | 2.450 * | 2.420 |
Rmerge | 0.076 | 0.269 |
Number of reflections | 43746 | |
<I/σ(I)> | 24.3 | 4 |
Completeness [%] | 95.0 | 96.4 |
Redundancy | 4.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | Logan, D.T., (1999) Science, 283, 1499. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | HEPES-NaOH | 0.1 (M) | pH7.5 |
2 | 1 | reservoir | 0.2 (M) | ||
3 | 1 | reservoir | dithiothreitol | 5-7 (mM) | |
4 | 1 | reservoir | PEG400 | 26-32 (%) | |
5 | 1 | drop | protein | 20-30 (mg/ml) |