1H76
The crystal structure of diferric porcine serum transferrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 223.770, 44.890, 78.860 |
| Unit cell angles | 90.00, 105.39, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.150 |
| R-factor | 0.136 * |
| Rwork | 0.136 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 0TFD |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.890 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0.32) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.310 * | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.073 | 0.123 |
| Total number of observations | 145143 * | |
| Number of reflections | 41503 | |
| <I/σ(I)> | 15.7 | 8.1 |
| Completeness [%] | 96.0 | 97.4 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8.5 | 277 * | pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 120 (mg/ml) | in water |
| 2 | 1 | 1 | PEG4000 | 20 (%(w/v)) | |
| 3 | 1 | 1 | 0.2 (M) | ||
| 4 | 1 | 1 | Tris-HCl | 0.1 (M) |
