1H71

Psychrophilic Protease from Pseudoalteromonas 'TAC II 18'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	294
Detector technology	IMAGE PLATE
Detector	MAC Science DIP-2030
Spacegroup name	P 21 21 21
Unit cell lengths	54.000, 57.550, 161.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.100
Rwork	0.164
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kap
RMSD bond length	0.017 *
RMSD bond angle	3.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.070
Number of reflections	29234
Completeness [%]	96.7	97.7
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	4 or 10 *	Villeret, V., (1997) Protein Sci., 6, 2462. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	5-10 (%)
2	1	reservoir		1 (mM)
3	1	reservoir	Tris-HCl	100 (mM)	pH8.
4	1	drop	protein	20 (mg/ml)