1H6U

Internalin H: crystal structure of fused N-terminal domains.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-07-03
Detector	RAXIS IV++
Spacegroup name	I 21 21 21
Unit cell lengths	57.481, 100.682, 119.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.700 - 1.800
R-factor	0.191 *
Rwork	0.167
R-free	0.24000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d0b
RMSD bond length	0.025
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	1.660
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.060 *	0.321 *
Number of reflections	32149 *
<I/σ(I)>	22	7.42
Completeness [%]	98.4 *	96.5 *
Redundancy	3.2 *	2.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sparse matrix method *	7 *	293	10% PEG 8000, 0.1M TRIS PH 7.0, 0.2 M MGCL2. T=20C, PROTEIN CONC.=10 MG/ML

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	10 (%)
2	1	1	Tris	0.1 (M)
3	1	1		0.2 (M)