1H6P
Dimeristion domain from human TRF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.041, 80.284, 52.816 |
| Unit cell angles | 90.00, 101.95, 90.00 |
Refinement procedure
| Resolution | 23.720 - 2.200 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.26800 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 17.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.630 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.052 | 0.231 |
| Total number of observations | 89685 * | |
| Number of reflections | 24415 | |
| <I/σ(I)> | 9.8 | 3.1 |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | PH7.0, 15-20% PEG 8000, 100-200 MM MAGNESIUM ACETATE, pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | glycerol | 4 (%) | |
| 4 | 1 | drop | PMSF | 0.4 (mM) | |
| 5 | 1 | drop | mercaptoethanol | 4 (mM) | |
| 6 | 1 | drop | protein | 20 (mg/ml) | |
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 8 | 1 | reservoir | PEG8000 | 15-20 (%) | |
| 9 | 1 | reservoir | 100-200 (mM) |
