1H6J
The three-dimensional structure of capsule-specific CMP:2-keto-3-deoxy-manno-octonic acid synthetase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 297 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.090, 133.460, 48.330 |
Unit cell angles | 90.00, 102.51, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.320 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.26600 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.068 |
Number of reflections | 22560 |
<I/σ(I)> | 13 |
Completeness [%] | 95.0 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 9.4 | 20 * | pH 9.40 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG20000 | 16 (%) | |
2 | 1 | drop | Tris-HCl | 100 (mM) | |
3 | 1 | drop | cadmium acetate | 200 (mM) | |
4 | 1 | drop | 5 (mM) | ||
5 | 1 | drop | dithiothreitol | 2 (mM) | |
6 | 1 | reservoir | PEG20000 | 2-4 (%) |