1H6H
Structure of the PX domain from p40phox bound to phosphatidylinositol 3-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-15 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.760, 33.642, 76.430 |
Unit cell angles | 90.00, 132.42, 90.00 |
Refinement procedure
Resolution | 30.730 - 1.700 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.23200 |
Structure solution method | MIR |
RMSD bond length | 0.012 |
RMSD bond angle | 21.400 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.400 | 1.770 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.084 | 0.156 |
Total number of observations | 55546 * | |
Number of reflections | 18117 | |
<I/σ(I)> | 6.7 | 4.2 |
Completeness [%] | 89.3 | 50.6 |
Redundancy | 3.1 | 1.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 17 * | used hair seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.9 (mg/ml) | |
2 | 1 | drop | di-C4-PtdIns(3)P | 2 (mM) | |
3 | 1 | reservoir | PEG6000 | 10 (%) | |
4 | 1 | reservoir | HEPES | 100 (mM) | |
5 | 1 | reservoir | 100 (mM) | ||
6 | 1 | reservoir | glycerol | 15 (%) |