1H6H

Structure of the PX domain from p40phox bound to phosphatidylinositol 3-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-15
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	96.760, 33.642, 76.430
Unit cell angles	90.00, 132.42, 90.00

Refinement procedure

Resolution	30.730 - 1.700
R-factor	0.196
Rwork	0.196
R-free	0.23200
Structure solution method	MIR
RMSD bond length	0.012
RMSD bond angle	21.400 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.400	1.770
High resolution limit [Å]	1.700	1.700
Rmerge	0.084	0.156
Total number of observations	55546 *
Number of reflections	18117
<I/σ(I)>	6.7	4.2
Completeness [%]	89.3	50.6
Redundancy	3.1	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	17 *	used hair seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8.9 (mg/ml)
2	1	drop	di-C4-PtdIns(3)P	2 (mM)
3	1	reservoir	PEG6000	10 (%)
4	1	reservoir	HEPES	100 (mM)
5	1	reservoir		100 (mM)
6	1	reservoir	glycerol	15 (%)