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1H6H

Structure of the PX domain from p40phox bound to phosphatidylinositol 3-phosphate

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSRS BEAMLINE PX9.6
Synchrotron siteSRS
BeamlinePX9.6
Temperature [K]100
Detector technologyCCD
Collection date2001-05-15
DetectorADSC CCD
Spacegroup nameC 1 2 1
Unit cell lengths96.760, 33.642, 76.430
Unit cell angles90.00, 132.42, 90.00
Refinement procedure
Resolution30.730 - 1.700
R-factor0.196
Rwork0.196
R-free0.23200
Structure solution methodMIR
RMSD bond length0.012
RMSD bond angle21.400

*

Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareSOLVE
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]56.4001.770
High resolution limit [Å]1.7001.700
Rmerge0.0840.156
Total number of observations55546

*

Number of reflections18117
<I/σ(I)>6.74.2
Completeness [%]89.350.6
Redundancy3.11.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

7.517

*

used hair seeding

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein8.9 (mg/ml)
21dropdi-C4-PtdIns(3)P2 (mM)
31reservoirPEG600010 (%)
41reservoirHEPES100 (mM)
51reservoir100 (mM)
61reservoirglycerol15 (%)

227111

PDB entries from 2024-11-06

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