1H6G

alpha-catenin M-domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-09-08
Detector	ADSC IMAGE PLATE
Spacegroup name	P 1 21 1
Unit cell lengths	42.863, 85.703, 75.481
Unit cell angles	90.00, 100.47, 90.00

Refinement procedure

Resolution	24.740 - 2.200
R-factor	0.193
Rwork	0.193
R-free	0.25700
Structure solution method	MAD
RMSD bond length	0.015 *
RMSD bond angle	1.510 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.049	0.205
Total number of observations	171946 *
Number of reflections	27803
<I/σ(I)>	13.3
Completeness [%]	99.1	98.4
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6	20 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	30 (%)
2	1	reservoir	sodium acetate	0.1 (M)
3	1	reservoir		20 (mM)