1H6B
Reduced Precursor Form of Glucose-Fructose Oxidoreductase from Zymomonas mobilis complexed with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.800, 92.774, 115.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ofg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.104 | 0.312 |
| Number of reflections | 27470 | |
| <I/σ(I)> | 6.1 | 2.4 |
| Completeness [%] | 96.9 | 83.9 |
| Redundancy | 5.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.4 * | 16% PEG6000, 5% GLYCEROL, 100MM K-SUCCINATE PH6.5, 1.5M SORBITOL, pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | MES/KOH | 40 (mM) | pH6.4 |
| 3 | 1 | reservoir | PEG6000 | 16 (%) | |
| 4 | 1 | reservoir | potassium succinate | 100 (mM) | pH6.5 |
| 5 | 1 | reservoir | glycerol | 5 (%) | |
| 6 | 1 | reservoir | sorbitol | 1.5 (M) |
