1H65
Crystal structure of pea Toc34 - a novel GTPase of the chloroplast protein translocon
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1998-06-15 |
Detector | ADSC CCD |
Wavelength(s) | 0.9796, 0.9794, 0.9680, 0.9802 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.160, 78.673, 67.279 |
Unit cell angles | 90.00, 91.44, 90.00 |
Refinement procedure
Resolution | 31.000 * - 2.000 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.23000 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 21.900 * |
Data reduction software | DPS |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.056 | 0.223 |
Number of reflections | 50147 | |
<I/σ(I)> | 8 | 1.9 |
Completeness [%] | 99.0 | 98.8 |
Redundancy | 7 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | THE TOC34 CRYSTALS WERE GROWN AT 4 DEGREES CELSIUS BY HANGING DROP VAPOR DIFFUSION. A 2-MICROLITER SOLUTION (10 MG/ML IN 50 MM TRIS-HCL AT PH 8.0 AND 0.1 M NACL) WAS MIXED WITH A 2-MICROLITER RESERVOIR SOLUTION CONTAINING 22% PEGMME 5K AND 10% GLYCEROL IN 0.1 M HEPES AT PH 6.5. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | pH8.0 |
3 | 1 | drop | 0.1 (M) | ||
4 | 1 | reservoir | PEG5000 MME | 22 (%(w/v)) | |
5 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
6 | 1 | reservoir | HEPES | 0.1 (M) | pH6.5 |