1H65

Crystal structure of pea Toc34 - a novel GTPase of the chloroplast protein translocon

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (2 Å)

Cell axes143.16078.67367.279
Cell angles90.0091.4490.00
SpacegroupC 1 2 1
Resolution limits22.42 - 2.00
the highest resolution shell value2.130 - 2.000
R-factor0.186
R-work0.18600
the highest resolution shell value0.229
R-free0.23000
the highest resolution shell value0.252
RMSD bond length0.009
RMSD bond angle1.200 (21.900*)

Data Collection Statistics

Resolution limits31.00 - 2.00
the highest resolution shell value -
Number of reflections50147
Rmerge_l_obs0.056
the highest resolution shell value0.223
Completeness99.0
Redundancy7
the highest resolution shell value2.5
I/sigma(I)2

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1VAPOR DIFFUSION, HANGING DROP6.5 (8.0*)277 (4*)*

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein10 (mg/ml)
21dropTris-HCl50 (mM)pH8.0
31drop0.1 (M)
41reservoirPEG5000 MME22 (%(w/v))
51reservoirglycerol10 (%(v/v))
61reservoirHEPES0.1 (M)pH6.5
Annotated Information is extracted from Literature Info*