1H64

CRYSTAL STRUCTURE OF THE SM-RELATED PROTEIN OF P. ABYSSI: THE BIOLOGICAL UNIT IS A HEPTAMER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-11-15
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	69.330, 70.160, 116.010
Unit cell angles	90.21, 97.70, 107.48

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.237
Rwork	0.237
R-free	0.28200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MODELLED HEPTAMER
RMSD bond length	0.007
RMSD bond angle	1.350 *
Data reduction software	XDS
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.049	0.147
Total number of observations	386356 *
Number of reflections	156396 *
<I/σ(I)>	14	4
Completeness [%]	96.2 *	95.2
Redundancy	2.3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	22 *	MPD, MAGNESIUM ACETATE, pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	27-29 (%)
2	1	reservoir	magnesium acetate	150 (mM)
3	1	reservoir	sodium cacodylate	50 (mM)	pH6.5