1H5B
T cell receptor Valpha11 (AV11S5) domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-15 |
| Detector | APS1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.531, 83.531, 132.463 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.830 - 1.850 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.24500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 26.700 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.340 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.044 | 0.541 |
| Number of reflections | 45847 | |
| <I/σ(I)> | 30.8 | 3.1 |
| Completeness [%] | 98.7 | 97.9 |
| Redundancy | 6.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 * | 20 * | 20C, VAPOR DIFFUSION, 3 UL PROTEIN (5 MG/ML IN 50 MM TRIS/CL, 100 MM NACL, PH 8.0) + 3 UL RESERVOIR SOLUTION (100 MM SODIUM CITRATE/HCL, 1.4-1.7 M LITHIUM CHLORIDE, PH 5.0-6.0) EQUILIBRATED AGAINST 1 ML OF RESERVOIR SOLUTION. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | sodium citrate-HCl | 100 (mM) | |
| 5 | 1 | reservoir | 1.4-1.7 (M) |
