1H56
Structural and biochemical characterization of a new magnesium ion binding site near Tyr94 in the restriction endonuclease PvuII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.180, 105.280, 46.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 3.000 |
R-factor | 0.15 |
Rwork | 0.150 |
R-free | 0.26400 * |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1pvu |
RMSD bond length | 0.007 |
RMSD bond angle | 22.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 8.000 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.130 |
Number of reflections | 8104 |
Completeness [%] | 92.3 |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | other * | 5 | Athanasiadis, A., (1991) J. Mol. Biol., 222, 451. * |