1H54
Maltose phosphorylase from Lactobacillus brevis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE D41A |
| Synchrotron site | LURE |
| Beamline | D41A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.750, 102.500, 114.000 |
| Unit cell angles | 90.00, 111.30, 90.00 |
Refinement procedure
| Resolution | 29.100 - 2.150 |
| R-factor | 0.173 * |
| Rwork | 0.186 |
| R-free | 0.21400 * |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.350 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.076 * | |
| Number of reflections | 95868 | |
| <I/σ(I)> | 23 | 7.7 |
| Completeness [%] | 98.2 | 95.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | O.1 M CITRATE, 0.03M PHOSPHATE; PH 6.0, 20% POLY ACRYLIC ACID 20000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8-10 (mg/ml) | |
| 2 | 1 | drop | citrate | 10 (mM) | |
| 3 | 1 | reservoir | PAA20000 | 18 (%) | |
| 4 | 1 | reservoir | citrate | 0.1 (M) | |
| 5 | 1 | reservoir | phosphate | 0.03 (M) |
