1H4R

Crystal Structure of the FERM domain of Merlin, the Neurofibromatosis 2 Tumor Suppressor Protein.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	87.018, 89.328, 96.764
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 * - 1.800
R-factor	0.193 *
Rwork	0.193
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1GC6.PDB
RMSD bond length	0.011
RMSD bond angle	1.390 *
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.0.36)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.065	0.622 *
Number of reflections	68222 *	6875 *
<I/σ(I)>	16.8	2.33
Completeness [%]	95.4 *	97.2
Redundancy	3.6 *	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5	294 *	THE PROTEIN WAS CRYSTALLIZED USING HANGING-DROP VAPOR DIFFUSION WIHTH 56% AMMONIUM SULFATE, 2% DIOXANE, 100 MM CACODYLATE, PH 6.5. A 1:1 RATIO OF PROTEIN TO WELL SOLUTION WAS USED.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	ammonium sulfate	56 (%saturated)
3	1	reservoir	dioxane	2 (%)
4	1	reservoir	sodium cacodylate	0.1 (M)