1H4P
Crystal structure of exo-1,3-beta glucanse from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-07-15 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 102.597, 102.597, 203.662 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 * - 1.750 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cz1 |
RMSD bond length | 0.006 |
RMSD bond angle | 22.000 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.0) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.043 | 0.443 * |
Total number of observations | 107423 * | |
Number of reflections | 104421 | |
<I/σ(I)> | 37 | 2.8 |
Completeness [%] | 97.6 * | 86.9 * |
Redundancy | 12 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 18 * | 100 MM SODIUM HEPES PH 7.8, 25 MM MGCL2, 1.3 M TRI-SODIUM CITRATE, 20% GLYCEROL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | trisodium citrate dihydrate | 1.2 (M) | |
3 | 1 | reservoir | HEPES | 100 (mM) | pH8. |
4 | 1 | reservoir | 25 (mM) |