1H4A
Human GammaD Crystallin R58H mutant structure AT 1.15 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.467, 53.234, 89.809 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.330 * - 1.150 |
R-factor | 0.1576 * |
Rwork | 0.155 |
R-free | 0.18220 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1elp |
RMSD bond length | 0.751 * |
RMSD bond angle | 1.453 * |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.330 | 1.210 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.050 | 0.109 |
Total number of observations | 463412 * | |
Number of reflections | 55087 | |
<I/σ(I)> | 9.2 | 6.3 |
Completeness [%] | 95.3 | 97.6 |
Redundancy | 4.6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 7 | 277 | 10MM PHOSPHATE, 4 DEGREE CELSIUS, pH 7.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | sodium phosphate | 100 (mM) | pH7. |
2 | 1 | 1 | dithiothreitol | 20 (ml) |