1H45

R210G N-TERMINAL LOBE HUMAN LACTOFERRIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	113
Detector technology	IMAGE PLATE
Detector	RIGAKU IMAGE PLATE
Spacegroup name	C 1 2 1
Unit cell lengths	131.720, 58.890, 57.530
Unit cell angles	90.00, 114.38, 90.00

Refinement procedure

Resolution	30.000 - 1.950
R-factor	0.222
Rwork	0.222
R-free	0.24700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lct
RMSD bond length	0.006
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.053	0.146
Number of reflections	27857
<I/σ(I)>	13.6	4.2
Completeness [%]	94.2	60.4
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	8	4 *	HEPES, NACL, pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	28 (mg/ml)
2	1	1	HEPES	20 (mM)
3	1	1		1 (M)	pH8.0