1H42
FERREDOXIN:NADP+ REDUCTASE MUTANT WITH THR 155 REPLACED BY GLY, ALA 160 REPLACED BY THR AND LEU 263 REPLACED BY PRO (T155G-A160T-L263P)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-11-15 |
Detector | MAR scanner 180 mm plate |
Spacegroup name | P 65 |
Unit cell lengths | 85.810, 85.810, 96.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.860 - 2.150 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1que |
RMSD bond length | 0.018 |
RMSD bond angle | 27.400 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.940 * | 2.270 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.095 | 0.287 |
Number of reflections | 21605 * | |
<I/σ(I)> | 0.095 | 0.287 |
Completeness [%] | 98.9 | 98.9 |
Redundancy | 6.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 20 * | pH 5.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
2 | 1 | drop | beta-octylglucoside | 5 (%(w/v)) | |
3 | 1 | reservoir | PEG6000 | 18-20 (%(w/v)) | |
4 | 1 | reservoir | ammonium sulfate | 20 (mM) | |
5 | 1 | reservoir | MES-NaOH | 0.1 (M) | pH5.0 |