1H41
Pseudomonas cellulosa E292A alpha-D-glucuronidase mutant complexed with aldotriuronic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.412, 74.393, 87.462 |
| Unit cell angles | 115.09, 93.04, 109.44 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.118 |
| Rwork | 0.118 |
| R-free | 0.15300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gqi |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 1.936 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.08) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.047 * | 0.237 * |
| Number of reflections | 212953 | 8276 * |
| <I/σ(I)> | 20.06 | 4.23 |
| Completeness [%] | 91.2 | 52.9 |
| Redundancy | 2.7 | 2.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 20 * | Nurizzo, D., (2002) Structure, 10, 547. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
| 3 | 1 | reservoir | 250 (mM) | ||
| 4 | 1 | reservoir | ethylene glycol | 20 (%(v/v)) | |
| 5 | 1 | reservoir | PEG3350 | 15 (%(w/v)) |
