1H3P
STRUCTURAL CHARACTERISATION OF A MONOCLONAL ANTIBODY SPECIFIC FOR THE PRES1 REGION OF THE HEPATITIS B VIRUS
Replaces: 1GM3Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE D41A |
| Synchrotron site | LURE |
| Beamline | D41A |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.010, 88.460, 111.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.2126 |
| Rwork | 0.213 |
| R-free | 0.29080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h1l |
| RMSD bond length | 0.005 |
| RMSD bond angle | 29.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.581) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.077 * | 0.272 |
| Number of reflections | 14823 | |
| <I/σ(I)> | 9.8 | 5.6 |
| Completeness [%] | 98.3 | 88 |
| Redundancy | 6.5 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 17 * | 0.1M CYSTEINE, 15% PEG6000, 110MM MGCL2, 60MM TRISHCL PH8.5, 15% GLYCEROL, pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | cysteine | 0.1 (M) | |
| 2 | 1 | reservoir | PEG6000 | 15 (%(w/v)) | |
| 3 | 1 | reservoir | 110 (mM) | ||
| 4 | 1 | reservoir | Tris-HCl | 60 (mM) | pH8.5 |
| 5 | 1 | reservoir | glycerol | 15 (%) | |
| 6 | 1 | drop | protein | 4.7 (mg/ml) |
