1H3O

Crystal Structure of the Human TAF4-TAF12 (TAFII135-TAFII20) Complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-09
Detector	MARRESEARCH
Wavelength(s)	0.90730,0.97740
Spacegroup name	C 1 2 1
Unit cell lengths	112.940, 36.825, 73.948
Unit cell angles	90.00, 97.85, 90.00

Refinement procedure

Resolution	20.000 * - 2.300
R-factor	0.226
Rwork	0.226
R-free	0.27200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	18.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.035 *	0.122 *
Total number of observations	61754 *
Number of reflections	25898 *	2588 *
<I/σ(I)>	19.9	4.45
Completeness [%]	98.9 *	99.7 *
Redundancy	1.5	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.25	24 *	pH 5.25

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	dithiothreitol	5 (mM)
2	1	drop	protein	10-15 (mg/ml)
3	1	reservoir	sodium acetate	100 (mM)	pH5.25
4	1	reservoir	ammonium sulfate	2.2 (M)