1H38
Structure of a T7 RNA polymerase elongation complex at 2.9A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.907, 84.971, 202.003 |
| Unit cell angles | 90.36, 92.97, 109.94 |
Refinement procedure
| Resolution | 40.000 * - 2.900 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qln |
| RMSD bond length | 0.016 |
| RMSD bond angle | 21.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.690 |
| High resolution limit [Å] | 2.900 * | 2.900 * |
| Rmerge | 0.076 * | 0.335 * |
| Total number of observations | 348602 * | |
| Number of reflections | 108303 * | |
| <I/σ(I)> | 14.7 | 2.9 |
| Completeness [%] | 98.1 * | 94.5 * |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.1 | 20 * | 10% PEG 8000, 8% GLYCEROL, 5MM BETA-MERCAPTOETHANOL,100 MM TRIS BUFFER, PH8.1, pH 8.10 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 10 (%) | |
| 3 | 1 | reservoir | glycerol | 10 (%) | |
| 4 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) | |
| 5 | 1 | reservoir | Tris | 100 (mM) | pH8.1 |
