1H30
C-terminal LG domain pair of human Gas6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-15 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 111.310, 111.310, 217.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.25900 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.082 | 0.297 |
| Number of reflections | 35018 | |
| <I/σ(I)> | 14.8 | 3.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium HEPES | 0.1 (M) | pH7.5 |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
| 6 | 1 | reservoir | 1.4-1.5 (M) |
