1H2W
PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-05-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.600, 100.100, 111.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.000 - 1.390 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qfm |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 1.440 |
High resolution limit [Å] | 1.390 | 1.390 |
Rmerge | 0.060 | 0.747 |
Total number of observations | 414442 * | |
Number of reflections | 144474 | |
<I/σ(I)> | 16.4 | 1.5 |
Completeness [%] | 89.6 | 76 |
Redundancy | 2.9 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | 4. * | Fulop, V., (1998) Cell, 94, 161. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | mPEG5000 | 17 (%(w/v)) | |
2 | 1 | reservoir | glycerol | 15 (%(v/v)) | |
3 | 1 | reservoir | monothioglycerol | 1 (%(v/v)) | |
4 | 1 | reservoir | 20 (mM) | ||
5 | 1 | reservoir | Tris | 100 (mM) | |
6 | 1 | drop | protein | 10 (mg/ml) |