1H2S
Molecular basis of transmenbrane signalling by sensory rhodopsin II-transducer complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 124.300, 46.960, 53.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.000 - 1.930 |
| R-factor | 0.2255 |
| Rwork | 0.226 |
| R-free | 0.25800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jgj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.070 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | |
| High resolution limit [Å] | 1.930 | |
| Rmerge | 0.062 | 0.394 * |
| Number of reflections | 23156 | |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 94.7 | 77.3 * |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.1 | 22 * | 150 MM NACL, 25 MM NAKPI 5.1 0.8% B-OCTYLGLUCOSID , MONOVACCENIN (CUBIC PHASE) PRECIPITATED BY 1 M NA/KPI 5.8 AT 22 C, pH 5.10 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 150 (mM) | ||
| 2 | 1 | 1 | sodium potassium Pi | 25 (mM) | pH5.1 |
| 3 | 1 | 1 | n-octyl-beta-D-glucopyranoside | 0.8 (%) |
