1H2H
Crystal structure of TM1643
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Collection date | 2002-07-15 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.196, 63.196, 125.673 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 * - 2.600 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.29100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 * | 2.600 |
| High resolution limit [Å] | 2.600 * | 2.500 |
| Rmerge | 0.067 * | 0.126 |
| Total number of observations | 87706 * | |
| Number of reflections | 9409 | |
| <I/σ(I)> | 25 | 20 |
| Completeness [%] | 98.6 | 93.2 |
| Redundancy | 15 | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 0.2M KDIH.PHOSPHATE, 22%PEG1500, 2% ETHYLENE GLYCOL, pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | |
| 2 | 1 | drop | 500 (mM) | ||
| 3 | 1 | drop | protein | 20 (mg/ml) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | PEG1500 | 22 (%) | |
| 6 | 1 | reservoir | EDTA | 1 (mM) |
