1H2B
Crystal Structure of the Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Aeropyrum pernix at 1.65A Resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 100.661, 103.186, 67.521 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.620 |
| R-factor | 0.16829 |
| Rwork | 0.168 |
| R-free | 0.20330 * |
| Structure solution method | MAD |
| Starting model (for MR) | 1hsz |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 1.746 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.054 | 0.932 |
| Number of reflections | 88078 * | |
| <I/σ(I)> | 28.9 | 1.52 |
| Completeness [%] | 97.9 | 95.6 |
| Redundancy | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.75 | pH 6.75 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PIPES | 100 (mM) | pH6.75 |
| 2 | 1 | reservoir | PEG600 | 13-16 (%(w/v)) | |
| 3 | 1 | reservoir | NADH | 0.5 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
