1H2A
SINGLE CRYSTALS OF HYDROGENASE FROM DESULFOVIBRIO VULGARIS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 280 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.500, 126.500, 66.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.27800 |
| Structure solution method | MIR AND MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.440 * |
| Data reduction software | WEIS |
| Data scaling software | FSCALE |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.105 | |
| Total number of observations | 251414 * | |
| Number of reflections | 63133 * | |
| Completeness [%] | 81.3 | 46 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.4 * | Higuchi, Y., (1987) J.Biol.Chem., 262, 2823. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 40 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 25 (mM) | |
| 3 | 1 | 1 | 20 (mM) | ||
| 4 | 1 | 2 | PEG | 20 (%(w/v)) | |
| 5 | 1 | 2 | sodium citrate | 20 (%(w/v)) | |
| 6 | 1 | 2 | ammonium sulfate | 40 (%sat) | |
| 7 | 1 | 2 | ethanol | 40 (%(v/v)) | |
| 8 | 1 | 2 | MPD | 40 (%(v/v)) | or 1M NaCl |
