1H27
CDK2/CyclinA in complex with an 11-residue recruitment peptide from p27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.687, 133.547, 148.081 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 * - 2.200 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.26100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qmz |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.190 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.990 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.073 | 0.399 |
| Total number of observations | 181975 * | |
| Number of reflections | 63569 | |
| <I/σ(I)> | 6.4 | 1.4 |
| Completeness [%] | 98.7 | 98.7 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.4 * | 0.8M KCL, 1.2M (NH4)2SO4, 40MM HEPES PH 7.0. PROTIEN CONCENTRATION = 10MG/ML |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | pH7.4 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | EDTA | 3.4 (mM) | |
| 5 | 1 | drop | azide | 0.01 (%) | |
| 6 | 1 | drop | monothiglycerol | 0.01 (%) | |
| 7 | 1 | reservoir | 0.8 (M) | ||
| 8 | 1 | reservoir | ammonium sulfate | 1.2 (M) | |
| 9 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
