Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.350, 113.450, 159.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 3.000* |
| Rwork | 0.219 |
| R-free | 0.26800 |
| Structure solution method | MAD |
| Starting model (for MR) | 1DB0 |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.580 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 3.000 | |
| Rmerge | 0.074 | 0.300 |
| Number of reflections | 20076 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 98.0 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 4 * | Robinson, R.C., (1999) Science, 286, 1939. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
| 2 | 1 | drop | ATP | 0.2 (mM) | |
| 3 | 1 | drop | 0.1 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 5 | 1 | drop | protein | 10 (mg/ml) | |
| 6 | 1 | reservoir | PEG8000 | 10 (%) | |
| 7 | 1 | reservoir | glycerol | 20 (%) | |
| 8 | 1 | reservoir | HEPES | 100 (mM) |
