1H1O
Acidithiobacillus ferrooxidans cytochrome c4 structure supports a complex-induced tuning of electron transfer
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Collection date | 2000-05-15 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 100.049, 100.049, 149.668 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 * - 2.200* |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.28400 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 19.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 * | 2.200 |
| High resolution limit [Å] | 2.200 * | 2.130 |
| Rmerge | 0.041 * | 0.245 * |
| Total number of observations | 112983 * | |
| Number of reflections | 23012 * | |
| <I/σ(I)> | 8.2 | 2.3 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 4.9 | 5.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 2.9 * | MES 0.1M PH 6.5, PEG MME 30%, 10MM ZINC SULFATE, 5% GLYCEROL | |
| 1 | Vapor diffusion * | 2.9 * | MES 0.1M PH 6.5, PEG MME 30%, 10MM ZINC SULFATE, 5% GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | glycine-HCl | 20 (mM) | pH2.9 |
| 3 | 1 | drop | ascorbate | 1 (mM) | |
| 4 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
| 5 | 1 | reservoir | PEG MME | 25-30 (%) | |
| 6 | 1 | reservoir | zinc sulfate | 10 (mM) | |
| 7 | 1 | reservoir | ascorbate | 1 (mM) | |
| 8 | 1 | reservoir | glycerol | 5 (%) |
