1H1K

THE BLUETONGUE VIRUS (BTV) CORE BINDS DSRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	281
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	795.600, 821.800, 753.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 10.000
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.000 *	4.000
High resolution limit [Å]	10.000 *	3.500
Rmerge	0.100 *	0.600
Total number of observations	490727 *
Number of reflections	251202 *
<I/σ(I)>	2.9	0.72
Completeness [%]	94.7 *	7.8
Redundancy	6.5	1.06

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8		Diprose, J.M., (2001) EMBO J., 20, 7229. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	25 (%sat)
2	1	reservoir	ethylene glycol	25 (%)
3	1	reservoir	Tris-HCl	0.1 (M)	pH8.0