1H1K
THE BLUETONGUE VIRUS (BTV) CORE BINDS DSRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID2 |
Synchrotron site | ESRF |
Beamline | ID2 |
Temperature [K] | 281 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 795.600, 821.800, 753.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | ? - 10.000 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.000 * | 4.000 |
High resolution limit [Å] | 10.000 * | 3.500 |
Rmerge | 0.100 * | 0.600 |
Total number of observations | 490727 * | |
Number of reflections | 251202 * | |
<I/σ(I)> | 2.9 | 0.72 |
Completeness [%] | 94.7 * | 7.8 |
Redundancy | 6.5 | 1.06 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8 | Diprose, J.M., (2001) EMBO J., 20, 7229. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 25 (%sat) | |
2 | 1 | reservoir | ethylene glycol | 25 (%) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |