1H1C
Histidinol-phosphate aminotransferase (HisC) from Thermotoga maritima
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9778,0.9862,0.9184 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 189.666, 146.168, 54.292 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.850 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.27200 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.944 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE/RESOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.092 | 0.381 |
| Number of reflections | 29624 | |
| <I/σ(I)> | 6.98 | 2.38 |
| Completeness [%] | 99.8 | 95.7 |
| Redundancy | 4.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | 20 * | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 2 | 1 | drop | dithiothreitol | 10 (mM) | |
| 3 | 1 | drop | EDTA | 2 (mM) | |
| 4 | 1 | drop | protein | 35 (mg/ml) | |
| 5 | 1 | reservoir | ethylene glycol | 40 (%(v/v)) | |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | pH6.2 |
