1H13
Structure of a cold-adapted family 8 xylanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 100 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.874, 90.509, 97.228 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.300 |
R-factor | 0.12 |
Rwork | 0.119 |
R-free | 0.14630 * |
Structure solution method | OTHER |
RMSD bond length | 0.022 |
RMSD bond angle | 1.811 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.039 | 0.326 |
Number of reflections | 111031 | |
<I/σ(I)> | 29.63 | 3.56 |
Completeness [%] | 99.2 | 99.3 |
Redundancy | 4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 * | 277 | Van Petegem, F., (2002) Acta Crystallogr., D58, 1494. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2 (mg/ml) | |
2 | 1 | reservoir | MPD | 70 (%) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |