1H0Y
Structure of Alba: an archaeal chromatin protein modulated by acetylation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 84.680, 84.680, 87.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h0x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.068 | 0.447 * |
| Number of reflections | 4115 | |
| <I/σ(I)> | 15.6 | 2.4 |
| Completeness [%] | 100.0 | 99.3 * |
| Redundancy | 23.3 * | 23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | Wardleworth, B.N., (2001) Acta Crystallogr.,Sect.D, 57, 1893. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 300 (mM) | ||
| 4 | 1 | reservoir | PEG8000 | 18 (%) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
| 6 | 1 | reservoir | 0.2 (M) | ||
| 7 | 1 | reservoir | 1,2,3-heptanetriol | 0.1 (M) |
