1H0K
Enoyl thioester reductase 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.546, 100.383, 80.852 |
| Unit cell angles | 90.00, 101.20, 90.00 |
Refinement procedure
| Resolution | 23.380 - 2.110 |
| R-factor | 0.152 |
| Rwork | 0.150 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gu7 |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.12) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.600 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.036 | 0.056 |
| Number of reflections | 57393 | |
| <I/σ(I)> | 22 | 15.4 |
| Completeness [%] | 95.6 | 91.2 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.00 |
