1H0J
Structural Basis of the Membrane-induced Cardiotoxin A3 Oligomerization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.902, 76.202, 47.784 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.27750 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tgx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 25.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.046 | 0.266 |
| Total number of observations | 93866 * | |
| Number of reflections | 21036 | |
| <I/σ(I)> | 28.5 | 3.91 |
| Completeness [%] | 94.4 | 96.3 |
| Redundancy | 4.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | 10 MG/ML PROTEIN MIXED WITH 0.1M SODIUM ACETATE (PH 4.6), 20% PEG 400, 3% GLYCEROL, AND 24 MM SDS. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
| 3 | 1 | reservoir | PEG400 | 20 (%) | |
| 4 | 1 | reservoir | glycerol | 3 (%) | |
| 5 | 1 | reservoir | SDS | 24 (mM) |
