1H0C

The crystal structure of human alanine:glyoxylate aminotransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-15
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	90.330, 90.330, 142.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.500
R-factor	0.231
Rwork	0.231
R-free	0.28600
Structure solution method	OTHER
RMSD bond length	0.014
RMSD bond angle	1.700
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE/RESOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.580
High resolution limit [Å]	2.500	2.500
Rmerge	0.074	0.177
Total number of observations	161988 *
Number of reflections	21044
<I/σ(I)>	6.9	3.8
Completeness [%]	99.9	99.9
Redundancy	7.7	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5	293 *	Zhang, X., (2001) Acta Crystallogr., D57, 1936. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15 (mg/ml)
2	1	1	PEG4000	10 (%)
3	1	1	sodium HEPES	0.1 (M)	pH7.5