1H0A
Epsin ENTH bound to Ins(1,4,5)P3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-15 |
Detector | ADSC CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 41.209, 95.823, 118.151 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.870 * - 1.700 |
R-factor | 0.189 |
Rwork | 0.185 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1edu |
RMSD bond length | 0.033 * |
RMSD bond angle | 2.300 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.870 * | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.093 | 0.558 |
Number of reflections | 24504 | |
<I/σ(I)> | 5.9798 | 1.33 |
Completeness [%] | 98.0 * | 89 * |
Redundancy | 5.9 * | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.4 | 35-38% DIOXANE, pH 7.40 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | dioxane | 35-38 (%) | |
2 | 1 | reservoir | Ins(a,4,5)P3 | 1.8 (mM) | |
3 | 1 | reservoir | epsinENTH | 0.600 (mM) |